Cell Counting - some useful information

Hi all,

Here's some really useful information that we received today about cell counting, that could make life easier if you are working with samples containing debris.

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Have a great day :)
Cellwyn

Using Trypan Blue and Fluorescent AOPI Dyes to Measure Cell Viability by Cellometer Cell Counters Do you have red-blood cells, cell debris, or tissue debris in your samples? Here are some samples that may contain RBCs, cell and tissue debris, and have been analyzed by the Cellometer instruments:     ·        Tail Vein Blood ·        Splenocytes ·        Bronchoalveolar lavage ·        Tumor Digests ·        PBMC ·        Lymphocytes ·        Cord Blood ·        Peripheral Blood ·        And many more   When your samples contain unwanted particles use: Acridine Orange/ Propidium Iodide (AO/PI) viability assay to accurately measure the concentration and viability of your samples!     AO/PI Protocol 1.     Obtain Nexcelom AO/PI solution: Cat # CS2-0106-5ML 2.     Stain cell sample at 1:1 with AO/PI solution 3.     Load counting chamber slide with 20 µl of cells and analyze Accurate and Consistent Results in <30 seconds!   To determine which instrument is best for you, contact a Nexcelom specialist today! Do you have clean cell samples without RBCs, or other cell debris? Here are some samples that may be considered clean samples, and have been analyzed by the Cellometer instruments:     ·        Over 1600 cultured cell lines ·        All NCI 60 cancer cell lines ·        Isolated Stem Cells ·        Isolated Mononuclear cells with RBC lysing ·        Frozen clinical Samples ·        Isolated Primary Cells   When your samples are free from unwanted particles use: Trypan Blue exclusion assay to accurately measure the concentration and viability of your samples!     Trypan Blue Protocol 1.     Dilute the stock (0.4 %) with PBS to 0.2 % 2.     Filter the trypan blue with 0.2 micron filter 3.     Mix the cell suspension at 1:1 with 0.2 % trypan blue 4.     Load the counting chamber slide with 20 µl of cells into the Cellometer and analyze Accurate and Consistent Results in <30 seconds!   To determine which instrument is best for you, contact a Nexcelom specialist today!